Journal: Brazilian Journal of Microbiology

Article Title: Characterization, modes of action, and application of a novel broad-spectrum bacteriocin BM1300 produced by Lactobacillus crustorum MN047

doi: 10.1007/s42770-020-00311-3

Figure Lengend Snippet: Antimicrobial spectrum of bacteriocin BM1300

Article Snippet: All measurements were performed in triplicate. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Indicator strains Indicator diameter of inhibition zone (mm) Resistant antibiotic Gram-positive bacteria Bacillus cereus ATCC 14579 25.8 ± 0.3 Staphylococcus aureus ATCC 26111 28.0 ± 0.0 Listeria monocytogenes ATCC 19115 21.6 ± 0.2 Listeria monocytogenes ATCC 15313 27 ± 1.0 Antibiotic-resistant Staphylococcus aureus 1 19.1 ± 1.0 AMP, A/C,T/S,PEN Antibiotic-resistant Staphylococcus aureus 2 16.3 ± 0.7 AMP, PEN, ERY, T/S, CIP Antibiotic-resistant Staphylococcus aureus 3 16.8 ± 0.3 AMP, PEN, T/S, CIP Antibiotic-resistant Staphylococcus aureus 4 0 ERY, FOX, A/C, T/S, PEN Antibiotic-resistant Staphylococcus aureus 5 0 ERY, FOX, AMP, A/C, T/S, PEN Gram-negative bacteria Salmonella typhimurium LT2 18.5 ± 0.3 Yersinia enterocolitica 52203 17.6 ± 0.2 Cronobacter sakazakii ATCC BAA-894 23.1 ± 0.3 Salmonella typhimurium ATCC 51005 17.6 ± 0.4 Vibrio parahaemolyticus ATCC 17802 13.5 ± 0.5 Antibiotic-resistant Salmonella 53-1105R 22.7 ± 0.3 TCY, FIS, TMP, SMZ Antibiotic-resistant Salmonella 1-1105J 15.9 ± 0.9 FIS, NAL Antibiotic-resistant Salmonella 557D 18.8 ± 0.3 AMP, A, AMK, CHL, FOX, GAT, GEN, KAN, LVX, NAL, T/S, TCY Antibiotic-resistant C . sakazakii 18-7 (1) 24.6 ± 0.2 AMO, A/C, TCY, S, RA, GEN, T/S, FOX Antibiotic-resistant C . sakazakii 18-11 (2) 15.0 ± 0.0 AMC, A/C, FOX, TCY, RA, T/S Antibiotic-resistant Escherichia coli SX DMQ004 EC2 18.9 ± 0.1 AMP, KAN, MP, SMZ, CHL, NAL, CIP, TET Antibiotic-resistant Escherichia coli SX WSQ003 EC3 18.2 ± 0.3 AMP, KAN, MP, SMZ, CHL, CFP, TET Open in a separate window A/C amoxicillin/clavulanic acid, AMC amoxicillin, AMP ampicillin, AMK amikacin, CHL chloramphenicol, CIP ciprofloxacin, CRO ceftriaxone, ERY erythromycin, FIS sulfisoxazole, FOX cefoxitin, GAT gatifloxacin, GEN gentamicin, KAN kanamycin, LVX levofloxacin, MP meropenem, NAL nalidixic acid, PEN penicillin, RA rifampin, S streptomycin, SMZ sulfamethoxazole, T/S trimethoprim/sulfamethoxazole, TCY tetracycline, TMP trimethoprim, TET tetracycline Antimicrobial spectrum of bacteriocin BM1300 Purification of BM1300 Crude bacteriocin BM1300 was dialyzed with a dialysis tube (MW 3500) in ultrapure water to remove salt.

Techniques: Inhibition

Journal: Antioxidants

Article Title: Resistance Training Modulates Reticulum Endoplasmic Stress, Independent of Oxidative and Inflammatory Responses, in Elderly People

doi: 10.3390/antiox11112242

Figure Lengend Snippet: Oxidative status, UPR IRE1 pathway activation, and inflammatory response at baseline in young and elderly subjects.

Article Snippet: Next, membranes were incubated with specific primary antibodies overnight at 4 °C [ ]: catalase (Ref. sc-50508), HSP60 (Ref. sc-376240), NRF2 (Ref. sc-722), SOD1 (Ref. sc-101523), SOD2 (Ref. sc-18503), and TLR4 (Ref. sc-293072) (Santa Cruz Biotechnology, Dallas, TX, USA); IRE1 (Ref. ab37073), and pIRE1 (Ref. ab48187) (Abcam); TRAF6 (Ref. 8028) and β-tubulin (Ref. 3700) (Cell Signaling Technology, Danvers, MA, USA); and pIRAK1 (Ref. OAAF00238) (AVIVA Systems Biology, San Diego, CA, USA).

Techniques: Activation Assay

Journal: Antioxidants

Article Title: Resistance Training Modulates Reticulum Endoplasmic Stress, Independent of Oxidative and Inflammatory Responses, in Elderly People

doi: 10.3390/antiox11112242

Figure Lengend Snippet: Representative Western blots showing the effects of aging (young, n = 11 (M); elderly, n = 30 (18/12 F/M)) ( A ) and resistance training (CG, n = 8 (7/1 F/M); TG, n = 22 (11/11 F/M)) ( B ) on catalase, NRF2, SOD1, SOD2, HSP60, Klotho, pIRE1, IRE1, pIRAK1, TLR4, and TRAF6 protein expression in PBMCs. CG, control group; F, female; M, male; TG, training group.

Article Snippet: Next, membranes were incubated with specific primary antibodies overnight at 4 °C [ ]: catalase (Ref. sc-50508), HSP60 (Ref. sc-376240), NRF2 (Ref. sc-722), SOD1 (Ref. sc-101523), SOD2 (Ref. sc-18503), and TLR4 (Ref. sc-293072) (Santa Cruz Biotechnology, Dallas, TX, USA); IRE1 (Ref. ab37073), and pIRE1 (Ref. ab48187) (Abcam); TRAF6 (Ref. 8028) and β-tubulin (Ref. 3700) (Cell Signaling Technology, Danvers, MA, USA); and pIRAK1 (Ref. OAAF00238) (AVIVA Systems Biology, San Diego, CA, USA).

Techniques: Western Blot, Expressing

Journal: Antioxidants

Article Title: Resistance Training Modulates Reticulum Endoplasmic Stress, Independent of Oxidative and Inflammatory Responses, in Elderly People

doi: 10.3390/antiox11112242

Figure Lengend Snippet: Oxidative status, UPR IRE1 pathway activation, and inflammatory response in the elderly at baseline and after an 8-week resistance training.

Article Snippet: Next, membranes were incubated with specific primary antibodies overnight at 4 °C [ ]: catalase (Ref. sc-50508), HSP60 (Ref. sc-376240), NRF2 (Ref. sc-722), SOD1 (Ref. sc-101523), SOD2 (Ref. sc-18503), and TLR4 (Ref. sc-293072) (Santa Cruz Biotechnology, Dallas, TX, USA); IRE1 (Ref. ab37073), and pIRE1 (Ref. ab48187) (Abcam); TRAF6 (Ref. 8028) and β-tubulin (Ref. 3700) (Cell Signaling Technology, Danvers, MA, USA); and pIRAK1 (Ref. OAAF00238) (AVIVA Systems Biology, San Diego, CA, USA).

Techniques: Activation Assay